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Santa Cruz Biotechnology integrin β1
Integrin β1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B21+integrin/Integrin+%CE%B21+Antibody/pm42886399-40-30-32
Average 96 stars, based on 894 article reviews

integrin β1 - by Bioz Stars, 2026-11

96/100 stars

Images

Saline:

Article Title: A-kinase anchoring protein 79/150 coordinates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor sensitization in sensory neurons.
Article Snippet: Samples isolated for co-immunoprecipitation were diluted to 500 μL with homogenization buffer10 and incubated with antibodies specific to AKAP150 (1 μg, Santa Cruz Biotechnology, clone E-1), pulled down with protein agarose-A (SigmaAldrich), and resolved by Sodium Dodecyl Sulfate -Polyacrylamide Gel Electrophoresis (SDS-PAGE). .. Gels were transferred to polyvinyldifluoride (PVDF, EMD Millipore), blocked in 5% non-fat milk in Tris buffered saline with 0.1% Tween-20 nonionic detergent, and incubated 18 h at 4°C with the following primary antibodies: AMPAGluR1 (Abcam ab19522), AMPA GluR2 (Abcam ab133477), AKAP150 (SantaCruz Biotechnology, clone E-1), SAP97 (Abcam, ab3437), β-arrestin (SigmaAldrich, clone 1B15), p-S831 AMPA GluR1 (Abcam, ab109464), p-S845 AMPA GluR1 (Abcam, ab76321), pS880 AMPA GluR2 (Abcam, ab52180), β1 integrin (SantaCruz Biotechnology, sc-374429). .. Anti-rabbit secondary antibodies (GE Healthcare Life Sciences) were applied to rinsed blots, incubated at RT for 1 h, and then blots were rinsed again.

Incubation:

Flow Cytometry:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Antibody , β1 integrin inhibiting (mouse monoclonal, clone 4B4LDC9LDH8 (4B4)) , Beckman Coulter , 41116015 , Functional assay: 5 μg/ml. .. Antibody , β1 integrin (mouse monoclonal, clone MEM-101A) , Santa Cruz Biotechnology , sc-51649 RRID: AB 629022 , Flow Cyt: 1:250 WB: ≈1:1000. .. Antibody , Vinculin (mouse monoclonal, clone hVIN-1) , Sigma , V9264 RRID: AB_10603627 , IF: 1:400-1:500 WB: 1:1000.

Western Blot:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Antibody , β1 integrin inhibiting (mouse monoclonal, clone 4B4LDC9LDH8 (4B4)) , Beckman Coulter , 41116015 , Functional assay: 5 μg/ml. .. Antibody , β1 integrin (mouse monoclonal, clone MEM-101A) , Santa Cruz Biotechnology , sc-51649 RRID: AB 629022 , Flow Cyt: 1:250 WB: ≈1:1000. .. Antibody , Vinculin (mouse monoclonal, clone hVIN-1) , Sigma , V9264 RRID: AB_10603627 , IF: 1:400-1:500 WB: 1:1000.

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Control siRNA pool (ON- TARGETplus siRNA, Dharmacon, D- 001810- 10- 05), collagen VI siRNA ON- TARGETplus siRNA (COL6A3, Human), SMARTPool, Horizon, L- 003646- 00- 0005. .. Antibodies were CD9 (SA35- 08 clone, NBP2- 67310, Novus Biologicals), CD63 (BD Pharmingen, 556019), CD81 (BD Pharmingen, 555676, B- 11, SantaCruz, sc- 166029 and M38 clone, NBP1- 44861, Novus Biologicals), Syntenin- 1 (Abcam, ab133267), Syndecan- 4 (Abcam, ab24511), α-Actinin- 4 (Abcam, ab108198), fibronectin (Abcam, ab2413, ab6328 [IST- 9] (3D matrix staining) and F14 clone, ab45688 (clinical samples analysis)), β1 activating (12G10) antibody was previously described (Parsons et al., 2008), 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma, V9264), α5 integrin (P1D6, Abcam, ab78614), Myo10 (Sigma, HPA024223), galectin- 3BP/MAC- 2BP (R&D systems, AF2226), EDIL3 antibody (R&D systems, MAB6046), TGFBI (Sigma, SAB2501486), IgG mouse (Sigma PP54), Anticollagen Type VI antibody, clone 3C4 (Sigma, MAB1944) (functional blocking), Anti- collagen Type VI antibody (Abcam, AB6588) (western blot), Anti- collagen Type VI antibody (Abcam, AB182744) (dot blot), p34- Arc/ARPC2 antibody (Millipore, #07–227), Cortactin (Abcam, ab81208 [EP1922Y]), GAPDH (ab139416, Abcam), Alix (Clone 3A9, ThermoFisher Scientific, MA1- 83977), EEA1 (BD Pharmingen, 610456), Elastin (Abcam, ab213720), β1 integrin (Santa Cruz Biotechnology, sc- 51649), β-actin (Sigma, A2228), Donkey Anti- mouse Secondary IRDye@680 LT (Li- COR, 926–68022) and Donkey Kapustin et al. eLife 2023;12:RP90375. .. DOI: https://doi.org/10.7554/eLife.90375 23 of 40 Anti- Rabbit Secondary IRDye@800 CW (Li- COR, 926–32213), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A21206), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A11008), AlexaFluor 488 anti- mouse IgG (Invitrogen, A21202), AlexaFluor 546 anti- rabbit IgG (Invitrogen, A10040), AlexaFluor 546 anti- mouse IgG (Invitrogen, A10036), AlexaFluor 647 anti- mouse IgG (Invitrogen, A31571), AlexaFluor 647 antirabbit IgG (Invitrogen, A31573), anti- mouse Alexa Fluor 488- conjugated IgG (Abcam, ab150109), anti- rabbit Alexa Fluor 555- conjugated IgG (Abcam, ab150062), anti- mouse IgG IRDye 680RD (LiCOR, 925–68070), anti- rabbit IgG IRDye@800 CW, goat polyclonal (Li- COR, 926–32211), horseradish peroxidase- conjugated anti- rabbit IgG (Cell Signaling Technology, 7074), horseradish peroxidaseconjugated anti- mouse IgG&IgM (Sigma- Aldrich, AP130P), horseradish peroxidase- conjugated anti- mouse IgG (GE Healthcare, NA931V), horseradish peroxidase- conjugated anti- rabbit IgG (GE Healthcare, NA934V), DNA plasmids were: CD63- GFP was kindly provided by Dr Aviva Tolkovsky (Bampton et al., 2005), CD63- pHluorin was previously described (Verweij et al., 2018), CD63- RFP was previously published (Leifer et al., 2004), Paxillin- RFP was previously described (Parsons et al., 2008), ARPC2–GFP DNA vector was a kind gift from Professor Michael Way laboratory (Abella et al., 2016), F- tractin- RFP was kindly provided by Dr Thomas S. Randall (King’s College London, UK), Myo10- GFP (Addgene Plasmid#135403) was previously described (Berg and Cheney, 2002).

other:

Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor binding
Article Snippet: The cell suspension was then washed, filtered with a 100-μm strainer (43-10100, pluriSelect) and centrifuged at 350 × g for 5 min at 4 °C.

Article Title: Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.
Article Snippet: Cells were seeded in 50-μl drops of Matrigel (356231, Corning).

Staining:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Control siRNA pool (ON- TARGETplus siRNA, Dharmacon, D- 001810- 10- 05), collagen VI siRNA ON- TARGETplus siRNA (COL6A3, Human), SMARTPool, Horizon, L- 003646- 00- 0005. .. Antibodies were CD9 (SA35- 08 clone, NBP2- 67310, Novus Biologicals), CD63 (BD Pharmingen, 556019), CD81 (BD Pharmingen, 555676, B- 11, SantaCruz, sc- 166029 and M38 clone, NBP1- 44861, Novus Biologicals), Syntenin- 1 (Abcam, ab133267), Syndecan- 4 (Abcam, ab24511), α-Actinin- 4 (Abcam, ab108198), fibronectin (Abcam, ab2413, ab6328 [IST- 9] (3D matrix staining) and F14 clone, ab45688 (clinical samples analysis)), β1 activating (12G10) antibody was previously described (Parsons et al., 2008), 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma, V9264), α5 integrin (P1D6, Abcam, ab78614), Myo10 (Sigma, HPA024223), galectin- 3BP/MAC- 2BP (R&D systems, AF2226), EDIL3 antibody (R&D systems, MAB6046), TGFBI (Sigma, SAB2501486), IgG mouse (Sigma PP54), Anticollagen Type VI antibody, clone 3C4 (Sigma, MAB1944) (functional blocking), Anti- collagen Type VI antibody (Abcam, AB6588) (western blot), Anti- collagen Type VI antibody (Abcam, AB182744) (dot blot), p34- Arc/ARPC2 antibody (Millipore, #07–227), Cortactin (Abcam, ab81208 [EP1922Y]), GAPDH (ab139416, Abcam), Alix (Clone 3A9, ThermoFisher Scientific, MA1- 83977), EEA1 (BD Pharmingen, 610456), Elastin (Abcam, ab213720), β1 integrin (Santa Cruz Biotechnology, sc- 51649), β-actin (Sigma, A2228), Donkey Anti- mouse Secondary IRDye@680 LT (Li- COR, 926–68022) and Donkey Kapustin et al. eLife 2023;12:RP90375. .. DOI: https://doi.org/10.7554/eLife.90375 23 of 40 Anti- Rabbit Secondary IRDye@800 CW (Li- COR, 926–32213), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A21206), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A11008), AlexaFluor 488 anti- mouse IgG (Invitrogen, A21202), AlexaFluor 546 anti- rabbit IgG (Invitrogen, A10040), AlexaFluor 546 anti- mouse IgG (Invitrogen, A10036), AlexaFluor 647 anti- mouse IgG (Invitrogen, A31571), AlexaFluor 647 antirabbit IgG (Invitrogen, A31573), anti- mouse Alexa Fluor 488- conjugated IgG (Abcam, ab150109), anti- rabbit Alexa Fluor 555- conjugated IgG (Abcam, ab150062), anti- mouse IgG IRDye 680RD (LiCOR, 925–68070), anti- rabbit IgG IRDye@800 CW, goat polyclonal (Li- COR, 926–32211), horseradish peroxidase- conjugated anti- rabbit IgG (Cell Signaling Technology, 7074), horseradish peroxidaseconjugated anti- mouse IgG&IgM (Sigma- Aldrich, AP130P), horseradish peroxidase- conjugated anti- mouse IgG (GE Healthcare, NA931V), horseradish peroxidase- conjugated anti- rabbit IgG (GE Healthcare, NA934V), DNA plasmids were: CD63- GFP was kindly provided by Dr Aviva Tolkovsky (Bampton et al., 2005), CD63- pHluorin was previously described (Verweij et al., 2018), CD63- RFP was previously published (Leifer et al., 2004), Paxillin- RFP was previously described (Parsons et al., 2008), ARPC2–GFP DNA vector was a kind gift from Professor Michael Way laboratory (Abella et al., 2016), F- tractin- RFP was kindly provided by Dr Thomas S. Randall (King’s College London, UK), Myo10- GFP (Addgene Plasmid#135403) was previously described (Berg and Cheney, 2002).

Functional Assay:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Control siRNA pool (ON- TARGETplus siRNA, Dharmacon, D- 001810- 10- 05), collagen VI siRNA ON- TARGETplus siRNA (COL6A3, Human), SMARTPool, Horizon, L- 003646- 00- 0005. .. Antibodies were CD9 (SA35- 08 clone, NBP2- 67310, Novus Biologicals), CD63 (BD Pharmingen, 556019), CD81 (BD Pharmingen, 555676, B- 11, SantaCruz, sc- 166029 and M38 clone, NBP1- 44861, Novus Biologicals), Syntenin- 1 (Abcam, ab133267), Syndecan- 4 (Abcam, ab24511), α-Actinin- 4 (Abcam, ab108198), fibronectin (Abcam, ab2413, ab6328 [IST- 9] (3D matrix staining) and F14 clone, ab45688 (clinical samples analysis)), β1 activating (12G10) antibody was previously described (Parsons et al., 2008), 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma, V9264), α5 integrin (P1D6, Abcam, ab78614), Myo10 (Sigma, HPA024223), galectin- 3BP/MAC- 2BP (R&D systems, AF2226), EDIL3 antibody (R&D systems, MAB6046), TGFBI (Sigma, SAB2501486), IgG mouse (Sigma PP54), Anticollagen Type VI antibody, clone 3C4 (Sigma, MAB1944) (functional blocking), Anti- collagen Type VI antibody (Abcam, AB6588) (western blot), Anti- collagen Type VI antibody (Abcam, AB182744) (dot blot), p34- Arc/ARPC2 antibody (Millipore, #07–227), Cortactin (Abcam, ab81208 [EP1922Y]), GAPDH (ab139416, Abcam), Alix (Clone 3A9, ThermoFisher Scientific, MA1- 83977), EEA1 (BD Pharmingen, 610456), Elastin (Abcam, ab213720), β1 integrin (Santa Cruz Biotechnology, sc- 51649), β-actin (Sigma, A2228), Donkey Anti- mouse Secondary IRDye@680 LT (Li- COR, 926–68022) and Donkey Kapustin et al. eLife 2023;12:RP90375. .. DOI: https://doi.org/10.7554/eLife.90375 23 of 40 Anti- Rabbit Secondary IRDye@800 CW (Li- COR, 926–32213), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A21206), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A11008), AlexaFluor 488 anti- mouse IgG (Invitrogen, A21202), AlexaFluor 546 anti- rabbit IgG (Invitrogen, A10040), AlexaFluor 546 anti- mouse IgG (Invitrogen, A10036), AlexaFluor 647 anti- mouse IgG (Invitrogen, A31571), AlexaFluor 647 antirabbit IgG (Invitrogen, A31573), anti- mouse Alexa Fluor 488- conjugated IgG (Abcam, ab150109), anti- rabbit Alexa Fluor 555- conjugated IgG (Abcam, ab150062), anti- mouse IgG IRDye 680RD (LiCOR, 925–68070), anti- rabbit IgG IRDye@800 CW, goat polyclonal (Li- COR, 926–32211), horseradish peroxidase- conjugated anti- rabbit IgG (Cell Signaling Technology, 7074), horseradish peroxidaseconjugated anti- mouse IgG&IgM (Sigma- Aldrich, AP130P), horseradish peroxidase- conjugated anti- mouse IgG (GE Healthcare, NA931V), horseradish peroxidase- conjugated anti- rabbit IgG (GE Healthcare, NA934V), DNA plasmids were: CD63- GFP was kindly provided by Dr Aviva Tolkovsky (Bampton et al., 2005), CD63- pHluorin was previously described (Verweij et al., 2018), CD63- RFP was previously published (Leifer et al., 2004), Paxillin- RFP was previously described (Parsons et al., 2008), ARPC2–GFP DNA vector was a kind gift from Professor Michael Way laboratory (Abella et al., 2016), F- tractin- RFP was kindly provided by Dr Thomas S. Randall (King’s College London, UK), Myo10- GFP (Addgene Plasmid#135403) was previously described (Berg and Cheney, 2002).

Blocking Assay:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Control siRNA pool (ON- TARGETplus siRNA, Dharmacon, D- 001810- 10- 05), collagen VI siRNA ON- TARGETplus siRNA (COL6A3, Human), SMARTPool, Horizon, L- 003646- 00- 0005. .. Antibodies were CD9 (SA35- 08 clone, NBP2- 67310, Novus Biologicals), CD63 (BD Pharmingen, 556019), CD81 (BD Pharmingen, 555676, B- 11, SantaCruz, sc- 166029 and M38 clone, NBP1- 44861, Novus Biologicals), Syntenin- 1 (Abcam, ab133267), Syndecan- 4 (Abcam, ab24511), α-Actinin- 4 (Abcam, ab108198), fibronectin (Abcam, ab2413, ab6328 [IST- 9] (3D matrix staining) and F14 clone, ab45688 (clinical samples analysis)), β1 activating (12G10) antibody was previously described (Parsons et al., 2008), 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma, V9264), α5 integrin (P1D6, Abcam, ab78614), Myo10 (Sigma, HPA024223), galectin- 3BP/MAC- 2BP (R&D systems, AF2226), EDIL3 antibody (R&D systems, MAB6046), TGFBI (Sigma, SAB2501486), IgG mouse (Sigma PP54), Anticollagen Type VI antibody, clone 3C4 (Sigma, MAB1944) (functional blocking), Anti- collagen Type VI antibody (Abcam, AB6588) (western blot), Anti- collagen Type VI antibody (Abcam, AB182744) (dot blot), p34- Arc/ARPC2 antibody (Millipore, #07–227), Cortactin (Abcam, ab81208 [EP1922Y]), GAPDH (ab139416, Abcam), Alix (Clone 3A9, ThermoFisher Scientific, MA1- 83977), EEA1 (BD Pharmingen, 610456), Elastin (Abcam, ab213720), β1 integrin (Santa Cruz Biotechnology, sc- 51649), β-actin (Sigma, A2228), Donkey Anti- mouse Secondary IRDye@680 LT (Li- COR, 926–68022) and Donkey Kapustin et al. eLife 2023;12:RP90375. .. DOI: https://doi.org/10.7554/eLife.90375 23 of 40 Anti- Rabbit Secondary IRDye@800 CW (Li- COR, 926–32213), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A21206), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A11008), AlexaFluor 488 anti- mouse IgG (Invitrogen, A21202), AlexaFluor 546 anti- rabbit IgG (Invitrogen, A10040), AlexaFluor 546 anti- mouse IgG (Invitrogen, A10036), AlexaFluor 647 anti- mouse IgG (Invitrogen, A31571), AlexaFluor 647 antirabbit IgG (Invitrogen, A31573), anti- mouse Alexa Fluor 488- conjugated IgG (Abcam, ab150109), anti- rabbit Alexa Fluor 555- conjugated IgG (Abcam, ab150062), anti- mouse IgG IRDye 680RD (LiCOR, 925–68070), anti- rabbit IgG IRDye@800 CW, goat polyclonal (Li- COR, 926–32211), horseradish peroxidase- conjugated anti- rabbit IgG (Cell Signaling Technology, 7074), horseradish peroxidaseconjugated anti- mouse IgG&IgM (Sigma- Aldrich, AP130P), horseradish peroxidase- conjugated anti- mouse IgG (GE Healthcare, NA931V), horseradish peroxidase- conjugated anti- rabbit IgG (GE Healthcare, NA934V), DNA plasmids were: CD63- GFP was kindly provided by Dr Aviva Tolkovsky (Bampton et al., 2005), CD63- pHluorin was previously described (Verweij et al., 2018), CD63- RFP was previously published (Leifer et al., 2004), Paxillin- RFP was previously described (Parsons et al., 2008), ARPC2–GFP DNA vector was a kind gift from Professor Michael Way laboratory (Abella et al., 2016), F- tractin- RFP was kindly provided by Dr Thomas S. Randall (King’s College London, UK), Myo10- GFP (Addgene Plasmid#135403) was previously described (Berg and Cheney, 2002).

Dot Blot:

Article Title: Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI
Article Snippet: Control siRNA pool (ON- TARGETplus siRNA, Dharmacon, D- 001810- 10- 05), collagen VI siRNA ON- TARGETplus siRNA (COL6A3, Human), SMARTPool, Horizon, L- 003646- 00- 0005. .. Antibodies were CD9 (SA35- 08 clone, NBP2- 67310, Novus Biologicals), CD63 (BD Pharmingen, 556019), CD81 (BD Pharmingen, 555676, B- 11, SantaCruz, sc- 166029 and M38 clone, NBP1- 44861, Novus Biologicals), Syntenin- 1 (Abcam, ab133267), Syndecan- 4 (Abcam, ab24511), α-Actinin- 4 (Abcam, ab108198), fibronectin (Abcam, ab2413, ab6328 [IST- 9] (3D matrix staining) and F14 clone, ab45688 (clinical samples analysis)), β1 activating (12G10) antibody was previously described (Parsons et al., 2008), 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma, V9264), α5 integrin (P1D6, Abcam, ab78614), Myo10 (Sigma, HPA024223), galectin- 3BP/MAC- 2BP (R&D systems, AF2226), EDIL3 antibody (R&D systems, MAB6046), TGFBI (Sigma, SAB2501486), IgG mouse (Sigma PP54), Anticollagen Type VI antibody, clone 3C4 (Sigma, MAB1944) (functional blocking), Anti- collagen Type VI antibody (Abcam, AB6588) (western blot), Anti- collagen Type VI antibody (Abcam, AB182744) (dot blot), p34- Arc/ARPC2 antibody (Millipore, #07–227), Cortactin (Abcam, ab81208 [EP1922Y]), GAPDH (ab139416, Abcam), Alix (Clone 3A9, ThermoFisher Scientific, MA1- 83977), EEA1 (BD Pharmingen, 610456), Elastin (Abcam, ab213720), β1 integrin (Santa Cruz Biotechnology, sc- 51649), β-actin (Sigma, A2228), Donkey Anti- mouse Secondary IRDye@680 LT (Li- COR, 926–68022) and Donkey Kapustin et al. eLife 2023;12:RP90375. .. DOI: https://doi.org/10.7554/eLife.90375 23 of 40 Anti- Rabbit Secondary IRDye@800 CW (Li- COR, 926–32213), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A21206), AlexaFluor 488 anti- rabbit IgG (Invitrogen, A11008), AlexaFluor 488 anti- mouse IgG (Invitrogen, A21202), AlexaFluor 546 anti- rabbit IgG (Invitrogen, A10040), AlexaFluor 546 anti- mouse IgG (Invitrogen, A10036), AlexaFluor 647 anti- mouse IgG (Invitrogen, A31571), AlexaFluor 647 antirabbit IgG (Invitrogen, A31573), anti- mouse Alexa Fluor 488- conjugated IgG (Abcam, ab150109), anti- rabbit Alexa Fluor 555- conjugated IgG (Abcam, ab150062), anti- mouse IgG IRDye 680RD (LiCOR, 925–68070), anti- rabbit IgG IRDye@800 CW, goat polyclonal (Li- COR, 926–32211), horseradish peroxidase- conjugated anti- rabbit IgG (Cell Signaling Technology, 7074), horseradish peroxidaseconjugated anti- mouse IgG&IgM (Sigma- Aldrich, AP130P), horseradish peroxidase- conjugated anti- mouse IgG (GE Healthcare, NA931V), horseradish peroxidase- conjugated anti- rabbit IgG (GE Healthcare, NA934V), DNA plasmids were: CD63- GFP was kindly provided by Dr Aviva Tolkovsky (Bampton et al., 2005), CD63- pHluorin was previously described (Verweij et al., 2018), CD63- RFP was previously published (Leifer et al., 2004), Paxillin- RFP was previously described (Parsons et al., 2008), ARPC2–GFP DNA vector was a kind gift from Professor Michael Way laboratory (Abella et al., 2016), F- tractin- RFP was kindly provided by Dr Thomas S. Randall (King’s College London, UK), Myo10- GFP (Addgene Plasmid#135403) was previously described (Berg and Cheney, 2002).

Review